Starting September 13, any oils and spreads that have trans fat will not be used by restaurants, delis, schools, hospitals and other businesses that serve food.
Mayor Thomas Menino said that this is a huge step towards healthier eating experiences in the city of Boston.
The ban was unanimously approved on Thursday by the city's Public Health Commission.
The fines put in place are set at $1,000 per violation of the trans fat ban.
"There are no health benefits and no level of consumption of artificial trans fat that is considered safe," said Dr. Paula Johnson, a cardiologist who chairs the commission's public health board.
Trans fats have been partly to blamed for raising "bad cholestrol," that clog arteries which increases the risk of heart disease such as heart attack and stroke.
